P- glycoprotein




WCCRB-2018
Scientific Federation invites all the participants from all over the world to attend 2nd World Congress on Clinical Research & Biomarkers during September 17-18, 2018 Toronto, Canada. 
Human natural killer cells are a population of large granular lymphocytes which can lyses tumor target cells in vitro without prior sensitization and with no major histocompatibility complex restriction. Lymph proliferative diseases of large granular lymphocytes can arise from T lymphocytes or natural killer cells. Large granular lymphocyte leukemia’s derived from T lymphocytes generally follow an indolent course with a median survival time exceeding many years. However, large granular leukemia’s involving natural killer cells are typically very aggressive and patients have a median survival time of two months due to being refractory to multi-agent chemotherapy. The World Health Organization classifies natural killer cell malignancies into three categories, including aggressive natural killer cell leukemia, extra nodal natural killer lymphoma and blastic natural killer cell lymphoma.
Though studies are sparse, natural killer cell malignancies seem to be relatively rare in Caucasians, but the incidence rises significantly in Asian and South American populations. One potentially promising approach is through the use of statins drugs. Statins have been used to lower LDL cholesterol levels in those with hypercholesterolemia and have been shown to have a limited number of side effects. Commonly prescribed statins include lovastatin, simvastatin, atorvastatin; fluvastatin, pravastatin, and rosuvastatin calcium. It shown that statins can have anti-inflammatory and immune modulator effects. In addition, statins may have potent anti-tumor properties.
In terms of the effect of statins on natural killer cell leukemias, a clinical case report noted that treatment with a farnesyl transferase inhibitor, which interrupts the mevalonate pathway farther downstream than the statins, resulted in clinical improvement and, importantly, a reduction of pulmonary artery hypertension. The pulmonary hypertension has been connected to damage to the pulmonary endothelial cells as a result of leukemic natural killer cell cytotoxicity. The novel effect of statins on leukemia cells is a new and potentially exciting field of research.

In the current research utilized the cell line YT-INDY to investigate the effect of statins on natural killer leukemic cells. The parental YT cell line is an interleukin-2-independent human leukemia natural killer cell line that has been used in numerous studies to investigate normal natural killer cell function. YT cells were isolated originally from a boy with acute lymphoblastic lymphoma. Investigations were performed because aggressive natural killer cell leukemias are devastating diseases which are nearly always fatal within weeks or, at best, a few months of diagnosis. Multi-agent chemotherapy has failed in the majority of patients with this disease. Therefore, new approaches to treatment must be developed that can provide a cure or significant life extension.

1 comments:

Lymphoma



WCCRB-2018
Scientific Federation invites all the participants from all over the world to attend 2nd World Congress on Clinical Research & Biomarkers during September 17-18, 2018 Toronto, Canada. 
There are many number of Lymphoma in pathology few of them are
Malignant lymphoma mainly includes Non-Hodgkin’s lymphoma (NHL) and Hodgkin’s lymphoma (HL). Its incidence is increasing and now ranges among the tenth most frequent cancers worldwide.

Mantle cell lymphoma (MCL) is an aggressive B-NHL that arises from naïve B cells in the mantle zone of the lymph nodes. It is characterized by translocation and subsequent over expression of CCND1.

Methylation study in Mantle cell lymphoma cell lines found in different expressed genes mapped to autosomal chromosomes. Pathway analysis revealed that top cellular functions represented cell death, cell cycle, cellular growth and proliferation. Mass ARRAY assay of 25 candidate genes in seven MCL cell lines and normal B cells showed genes hyper ethylated in more than one MCL cell line, encompassing cell differentiation, cell adhesion, cell cycle and apoptosis p53 pathway and PI3K pathway, transcription factors and many unknown functions are there in MCL

Hodgkin’s lymphoma (HL) Screening of methylated genes was performed in HL KM-H2 cell line by microarray analysis before and after treatment with 5-aza-2’-deoxycitidine. Thirty tumor suppressive genes were identified, including genes in cellular adhesion, growth arrest p53 pathway and two transcription factors. Among them was further confirmed methylated and down-regulated in primary HL cells. Restoration of CADM1 expression in HL cells decreased cell survival and increased their sensitivity to apoptosis, demonstrating that IGSF4silencing by CpG methylation inhibited apoptosis in Reed-Sternberg cells, which was an important process in HL pathogenesis.

0 comments:

Alpha-Fetoprotein







WCCRB-2018

Being an Organizing Committee Member at 2nd World Congress on Clinical Research & Biomarkers Conference held by Scientific Federation is invited as Speaker for this conference. Vladimir N. Pak has completed his PhD from Moscow Institute of Bioorganic Chemistry, Russia and has over 30 years of post-doctoral experience in virology, immunology, biotechnology, pharmacology and oncology. He has published 7 patents related to API research and medicines manufacturing. He is the inventor of the anti-cancer medicines based on alpha-fetoprotein (AFP)-toxin non-covalent complexes that act as a targeted chemotherapy and MDSCs-immunotherapy. As a freelance researcher I need to know what kind of discount you can provide to me.



Alpha-fetoprotein (AFP) is a pregnancy biomarker. The protein shuttles essential nutrients to AFP receptor (AFPR)-positive embryonic cells. For majority of cancers AFPR is a biomarker and it is also expressed by host myeloid-derived suppressor cells (MDSCs).AFP delivers selected nutrients to MDSCs that suppress innate and adaptive immunity during cancer, inflammation, hemopoiesis and regeneration.AFP loaded with a medicine can be helpful in treating autoimmune diseases and inflammation, in transplantation, etc. On the other hand, AFP loaded with a toxin can destroy cancer cells and MDSCs. MDSCs depletion unleashes innate and adaptive immunity to remove cancer cells. In addition to traditional use as a pregnancy and tumor biomarker AFP obtains the new immunotherapy drug use.

0 comments:

Lung Cancer


WCCRB-2018

Scientific Federation invites all the participants from all over the world to attend 2nd World Congress on Clinical Research & Biomarkers during September 17-18, 2018 Toronto, Canada.
Now a day’s cancer is very dangerous disease and an especially lung cancer plays major role. Blood based biomarkers have potential in cancer screening and their role could extend further from general population risk assessment to treatment response evaluation and recurrence monitoring. The rich content of diverse cellular and molecular elements in blood, which provide information about the health status of an individual, makes it an ideal compartment to develop noninvasive diagnostics for cancer. Other common cancers, notably breast and lung cancer, lack established biomarkers with demonstrated clinical utility in a screening setting. There is a need for biomarkers with the required sensitivity and specificity for the detection of frequently occurring cancer types. Protein markers currently in clinical use, which include Cancer antigen 125 for ovarian cancer, Carbohydrate antigen 199 for pancreatic cancer, Carcino embryonic antigen for colon cancer and Prostate specific antigen for prostate cancer, have limitations with respect to their use for screening owing to low sensitivity and specificity in early stages and inability to distinguish aggressive from indolent tumors.
This is significantly more sensitive than the most sensitive detection methods from electrophoretic gels with silver staining or fluorescence staining, or the general mass spectrometry methods. Secondly, it is known that many proteins have post-translational modifications (PTMs) and splice variants; these different forms of the same gene product may have different functions/enzyme activities and play very different roles in biology. However the important information on the impact of PTM and protein splicing is lost in the antibody, LC-MS or gel-based analysis because these platforms cannot directly measure the functional features of the proteome. The introduction of exogenous protein(s) from the PEP samples could potentially supersede any rate-limiting protein function and enhance the hexokinase activity. As such, this assay may also detect the effect of proteins from other pathways that cross-interact with the glycol tic pathway.

PS: http://scientificfederation.com/clinical-research-2018/

0 comments:

Subscribe to: Posts (Atom)